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Introduction Stroke remains a leading cause of adult disabil
2018-11-08
Introduction Stroke remains a leading cause of adult disability worldwide. In Brazil it is the leading cause of death, with a mortality rate of approximately 51.6 deaths per 100,000 inhabitants (Garcia et al., 2009) and shows the highest case-fatality rate in Latin America (Lotufo and Bensenor, 200
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Direct genetic reprogramming of postnatal primary cells by
2018-11-08
Direct genetic reprogramming of postnatal primary cells by forced expression of key developmental transcription factors has emerged as an alternative to in vitro differentiation of PSCs. This strategy has been used successfully to produce functional cells, including neurons, hepatocyte-like cells an
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The model of this current study showed that
2018-11-08
The model of this current study showed that CD133 and CD24 markers, as a single or combined expression, are so promiscuous that they are not able to identify a pure population of adult renal stem cells. In PKHhigh cells, the only cell population presenting self-renew and an in vitro multipotency cap
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To develop an effective cell
2018-11-08
To develop an effective cell-based therapy against neurodegenerative disorders of the CNS, efficient recruitment of the cells into the CNS is essential. Previous findings suggest that particular bone marrow derived cells are able to cross the blood–brain barrier (Lebson et al., 2010; Simard and Rive
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br Acknowledgments We thank all the
2018-11-08
Acknowledgments We thank all the members of Wagers laboratory and E. Gussoni and E. Dzierzak for helpful discussions. We also thank A. Wakabayashi, G. Buruzula and J. LaVecchio at Joslin/HSCI Flow Cytometry Core for excellent flow cytometry support, funded in part by NIHP30 DK036836. This work wa
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br Materials and methods br Results br Discussion This
2018-11-08
Materials and methods Results Discussion This present study and previous works in the literature have shown that mechanical stimulation promotes the proliferation of BMSCs (Luu et al., 2009). In this study, the presented results revealed that pressure promoted BMSC proliferation by facilita
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Our coculture experiments demonstrated that all three MSCs s
2018-11-08
Our coculture experiments demonstrated that all three MSCs showed a similar low in vitro proliferation; for most patients coculture of all three MSCs with primary human AML trp channels did not alter MSC proliferation but a minor increase in the proliferation was seen for a minority of patients. No
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ginsenoside rh2 This study was approved by
2018-11-08
This study was approved by the Institutional Stem Cell Research Oversight (ISCRO) committee in the Johns Hopkins University. Blood CD34+ ginsenoside rh2 were obtained with an informed consent from the patient. Materials and methods Acknowledgments We thank the patient for providing blood sam
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The WNT signaling pathway is a
2018-11-08
The WNT signaling pathway is a primary determinant in assigning an A/P positional identity to NPCs. This instructional cue is imparted early during NPC generation and once this identity is established, it is stable and cannot be altered through exogenous manipulation of the WNT pathway. Using a WNT
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The pleiotropic nature of FGF
2018-11-08
The pleiotropic nature of FGF2 and its variety of downstream effects make generation of highly pure active protein essential. Heparin chromatography has been used for purification of 18kDa FGF2 isoform. However, subsequent heparin contamination in purified FGF2 preparations has been previously descr
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br Materials and methods br Results
2018-11-08
Materials and methods Results Discussion NPY is one of the most common neuropeptides in mammals, and it is abundant in the central and peripheral nervous system. Recent studies have indicated that the neuropeptides SP and NPY can regulate many biological activities in BMSCs and MC3T3 src ki
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Neuroanatomical studies indicate that DARPP neurons
2018-11-08
Neuroanatomical studies indicate that DARPP32+ neurons could be divided into two populations: one that constructs a direct pathway and another that constructs an indirect pathway. DARPP32+ neurons in the direct pathway express DRD1 and are located in the striosome. On the other hand, those in the in
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The ability to use cell surface proteins to specifically sor
2018-11-07
The ability to use cell-surface proteins to specifically sort iPSC-derived hepatocyte-like cells is currently limited. The liver-specific cell-surface protein ASGR1 has been used to sort human ESC- and iPSC-derived hepatocyte-like cells (Basma et al., 2009; Peters et al., 2016). These studies demons
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While these studies differ somewhat
2018-11-07
While these studies differ somewhat in terms of their health measures and population samples, they all overlook a more fundamental backdrop: the changing nature of educational and health inequalities across the twentieth century. In the United States, high school and college education changed greatl
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Pandi Perumal and Gonfalone presented perspective on sleep
2018-11-07
Pandi-Perumal and Gonfalone presented perspective on sleep in space by overviewing the past medical reports . The authors made special emphasis on “normal sleep” in space, where no gravity exists. According to their review, sleep in space was mainly monitored by subjective sleep diaries and actigrap
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